Okokuqala, phambi kokukhetha i-drill bit, makhe sijonge ukuba yintoni na ubunzima ekwenzeni i-flange yensimbi engenasici? Fumana amanqaku anzima angachaneka kakhulu, ngokukhawuleza ukufumana ukusetyenziswa kwe-drill.ubunzima bokucutshungulwa kwe-flange yensimbi engenasici?Imela yentonga emfutshane: Intsimbi yokulungisa intsimbi yomelele kwaye iqinile, ubushushu bokusika buphezulu kakhulu. Ukukhanya komphezulu;Ukuqina komsebenzi kuqatha.
Ekusetyenzisweni kweeflange ezinkulu, kukho izinto ezininzi ezisebenzayo ezichaphazela iiflange ezinkulu.Oku kulandelayo zizinto eziliqela eziqhelekileyo.Eyokuqala yi-annealing temperatures (iqondo lobushushu le-annealing yiparameter yeqondo lobushushu le-primer kunye netemplate ngokulandelelanayo, kunye neqondo lobushushu xa i-50% ye-primer kunye nolandelelwano oluhambelanayo imelwe njenge-molekyuli ze-DNA eziphindwe kabini).

Yinto ebalulekileyo echaphazela ukuchaneka kwe-PCR.Kwimeko ye-aspiration, ukushisa kwe-annealing kuphantsi ngokwaneleyo ukuqinisekisa ukuba i-primer kunye nolandelelwano olujoliswe kuyo zombini ziluncedo kwaye ziphezulu ngokwaneleyo ukunciphisa ukwahlukana okungeyodwa.Iqondo lokushisa elifanelekileyo ukusuka kwi-55 ℃ ukuya kwi-70 ℃. Ubushushu be-annealing bubekwe ngokubanzi nge-5 ℃ ngaphantsi kwe-Tm yeeprimers.

Ukusuka:168 forgings net