Okokuqala, phambi kokukhetha i-drill bit, makhe sijonge ukuba yintoni na ubunzima ekwenzeni i-flange yensimbi engenasici? Fumana amanqaku anzima angachaneka kakhulu, ngokukhawuleza ukufumana ukusetyenziswa kwe-drill.ubunzima bokucutshungulwa kwe-flange yensimbi engenasici?Imela yentonga emfutshane: Itshiphu yokucoca intsimbi eyomeleleyo kwaye yomelele, ubushushu bokusika buphezulu kakhulu.Xa itshiphu eyomeleleyo neqinile inqumla kumphezulu wemela, iya kuhlasela iqhina, ukuwelda ukudibanisa kunye nezinye izinto ezidibanisayo, ezichaphazela iindawo zokusetyenzwa komphezulu wokuqaqamba;Ukuqina komsebenzi kuqatha.
Ekusetyenzisweni kweeflange ezinkulu, kukho izinto ezininzi ezisebenzayo ezichaphazela iiflange ezinkulu. Oku kulandelayo zizinto eziliqela eziqhelekileyo. Eyokuqala yi-annealing temperatures (iqondo lobushushu le-annealing yiparameter yeqondo lobushushu le-primer kunye netemplate ngokulandelelanayo, kunye neqondo lobushushu xa i-50% ye-primer kunye nolandelelwano oluhambelanayo imelwe njenge-molekyuli ze-DNA eziphindwe kabini).

Yinto ebalulekileyo echaphazela ukuchaneka kwe-PCR.Kwimeko yomnqweno, ukushisa kwe-annealing kuphantsi ngokwaneleyo ukuze kuqinisekiswe ukuba i-primer kunye nolandelelwano olujoliswe kuyo zombini ziluncedo kwaye ziphezulu ngokwaneleyo ukunciphisa ukwahlukana okungeyodwa.Iqondo lokushisa elichanekileyo lisuka kwi-55℃ ukuya kwi-70℃.I-annealing ye-annealing i-5m ye-annealing isethi jikelele ye-5 ℃ ye-primers.

Ukusuka:168 forgings net