Okokuqala, ngaphambi kokukhetha i-drill bit, ake sibheke ukuthi ibuphi ubunzima ekwenzeni i-flange yensimbi engagqwali?Thola amaphuzu anzima kungaba nenembile kakhulu, ngokushesha okukhulu ukuthola ukusetshenziswa kwe-drill.ubunzima bokucutshungulwa kwe-stainless steel flange?Ummese wenduku emfushane: i-stainless steel processing chip iqinile futhi iqinile, izinga lokushisa liphezulu kakhulu.Lapho i-chip eqinile neqinile igeleza endaweni engaphambili yommese, izohlasela i-bonding, i-fusion welding kanye nokunye okubophayo, okuthinta izingxenye zokucubungula ukukhanya okungaphezulu;Ukuqina komsebenzi kuqinile.
Ekusetshenzisweni kwama-flanges amakhulu, kunezici eziningi zokusebenza ezithinta ama-flange amakhulu.Okulandelayo yizici ezimbalwa ezivamile.Esokuqala izinga lokushisa le-annealing (izinga lokushisa le-annealing yipharamitha yezinga lokushisa le-primer nesifanekiso ngokulandelanayo, kanye nezinga lokushisa lapho u-50% we-primer nokulandelana okuhambisanayo emelelwa njengama-molecule e-DNA anemicu ekabili).

Kuyisici esibalulekile esithinta ukucaciswa kwe-PCR.Endabeni ye-aspiration, izinga lokushisa le-anneal liphansi ngokwanele ukuze kuqinisekiswe ukuthi i-primer kanye nokulandelana okuqondiwe kokubili kuyasiza futhi kuphakeme ngokwanele ukuze kuncishiswe ukuhlukana okungaqondile.Izinga lokushisa lokufafaza elinengqondo ibanga lisuka ku-55℃ liye ku-70℃.Izinga lokushisa le-anneal ngokuvamile lisethwa ngo-5℃ ngaphansi kwe-Tm yeziqalisi.

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