ʻO ka mea mua, ma mua o ke koho ʻana i ka drill bit, e nānā i nā pilikia o ka stainlesshili kilahana ʻana.E ʻike i ka paʻakikī hiki ke pololei loa, wikiwiki loa e ʻike i ka hoʻohana ʻana i ka drill.He aha nā pilikia i ka hana ʻana i ka flange kila?ʻO ka pahi paʻa: ʻo ka paʻakikī o ka ʻoki ʻoki ʻana i ka wela.Ke holo ka puʻupuʻu ikaika ma ka ʻili o ka ʻoki mua, e hoʻopili ʻia, fusion welding a me nā mea hoʻopili ʻē aʻe, e pili ana i ka ʻōlinolino o nā ʻāpana i hana ʻia;He paʻakikī ka hana.

I ka ai ana onā lehelehe nui, nui nā mea hana e pili ananā lehelehe nui.Eia mākou e kamaʻilio e pili ana i kekahi mau mea maʻamau.ʻO ka mua, ʻo ia ka mahana hoʻopulapula (ʻo ka mahana o ka annealing ka palena wela o ka primer a me ka template, inā ʻo 50% o ka primer a me ke kaʻina hoʻohui ʻia ka mahana o nā molekala DNA ʻelua.

He mea koʻikoʻi ia e pili ana i ka kikoʻī o PCR.I ka hihia o ka aspiration, he haʻahaʻa ka mahana annealing e hōʻoia i ka pale ʻana o ka primer me ke kaʻina pahuhopu, a me ka kiʻekiʻe e hōʻemi i nā kaʻawale nonspecific.ʻO nā mahana annealing kūpono mai 55 ℃ a 70 ℃.Hoʻonohonoho ʻia ka mahana annealing ma 5 ℃ haʻahaʻa ma mua o Tm o ka primer.