Da farko, kafin zabar rawar rawar jiki, duba matsalolin da ke cikin bakinkarfe flangesarrafawa.Gano wahalar na iya zama daidai sosai, da sauri don nemo amfani da rawar sojan.Menene matsaloli a cikin sarrafa bakin karfe flange?M wuka: bakin karfe sarrafa guntu taurin, yankan zafin jiki ne sosai high.Lokacin da guntu mai ƙarfi ke gudana ta saman gaban mai yankewa, zai faru da m, fusion waldi da sauran m sabon abu, wanda rinjayar da bayyanar haske na sarrafa sassa;Ƙarfafa aiki yana da tsanani.

A cikin cin abincimanyan flanges, akwai abubuwa masu aiki da yawa waɗanda ke tasirimanyan flanges.Anan muna magana game da abubuwa gama gari da yawa.Na farko shine zafin zafin jiki (zazzabi mai raɗaɗi shine ma'aunin zafin jiki na firamare da samfuri bi da bi, lokacin da 50% na firamare da madaidaitan jeri sune zazzabi na ƙwayoyin DNA mai ɗaure biyu.

Abu ne mai mahimmanci wanda ke shafar takamaiman PCR.A cikin yanayin sha'awa, zafin jiki mai raɗaɗi yana da ƙarancin isa don tabbatar da cewa an yanke matakin farko tare da jerin abubuwan da aka yi niyya, kuma yana da girma sosai don rage rarrabuwa na musamman.Madaidaicin yanayin zafi mai raɗaɗi daga 55 ℃ zuwa 70 ℃.Ana saita yawan zafin jiki na annealing a 5℃ ƙasa da Tm na farkon.